验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells, mouse skeletal muscle tissue, K-562 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, Transfected HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, human rectal cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ARHGEF7 Ready-To-Use IHC Kit. ARHGEF7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
14092-1-AP targets ARHGEF7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag5223 Product name: Recombinant human ARHGEF7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 455-803 aa of BC060776 Sequence: KELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRKSCCSYISHQN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho guanine nucleotide exchange factor (GEF) 7 |
| 别名 | KIAA0142, COOL-1, COOL1, COOL 1, Beta-Pix |
| 计算分子量 | 803 aa, 90 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC060776 |
| 基因名称 | ARHGEF7 |
| Gene ID (NCBI) | 8874 |
| RRID | AB_2227567 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14155 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ARHGEF7 antibody 14092-1-AP | Download protocol |
| IHC protocol for ARHGEF7 antibody 14092-1-AP | Download protocol |
| IP protocol for ARHGEF7 antibody 14092-1-AP | Download protocol |
| WB protocol for ARHGEF7 antibody 14092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7. | ||
Am J Transl Res Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3. | ||
J Control Release Dentin tubules as a long-term sustained release carrier to accelerate bone repair by loading FTY720 |
