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验证数据展示

  • WB analysis of mouse brain using 55213-1-AP (same clone as 55213-1-PBS)

    WB analysis of mouse brain using 55213-1-AP (same clone as 55213-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

  • WB analysis of multi-cells/tissue using 55213-1-AP (same clone as 55213-1-PBS)

    WB analysis of multi-cells/tissue using 55213-1-AP (same clone as 55213-1-PBS)

    WB result of 55213-1-AP (ARHGEF4 Antibody). This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 55213-1-AP (same clone as 55213-1-PBS)

    IHC staining of human brain using 55213-1-AP (same clone as 55213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 55213-1-AP (same clone as 55213-1-PBS)

    IHC staining of human brain using 55213-1-AP (same clone as 55213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 55213-1-AP (same clone as 55213-1-PBS)

    IP experiment of mouse brain using 55213-1-AP (same clone as 55213-1-PBS)

    IP result of anti-ARHGEF4 (IP:55213-1-AP, 4ug; Detection:55213-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ARHGEF4 Polyclonal antibody, PBS Only

ARHGEF4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 55213-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55213-1-PBS targets ARHGEF4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho guanine nucleotide exchange factor (GEF) 4
别名
计算分子量 79 kDa
观测分子量 72 kDa
GenBank蛋白编号NM_015320
基因名称 ARHGEF4
Gene ID (NCBI) 50649
RRIDAB_2721023
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR80
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.

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