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验证数据展示

  • IHC staining of human colon using 84149-1-RR

    IHC staining of human colon using 84149-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 84149-1-RR

    IHC staining of human colon using 84149-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HuH-7 using 84149-1-RR

    IF Staining of HuH-7 using 84149-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using ARHGEF11 antibody (84149-1-RR, Clone: 241427A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84149-1-RR

    Affinity and Kinetic Characterization of 84149-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84149-1-RR against Human ARHGEF11 were performed. The affinity constant is 1.09 nM.

ARHGEF11 Recombinant antibody

ARHGEF11 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84149-1-RR

产品说明书

CloneNo. 241427A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

241427A4, GTRAP48, KIAA0380, PDZ RHOGEF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHuH-7 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84149-1-RR targets ARHGEF11 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ARHGEF11 fusion protein Ag33104 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho guanine nucleotide exchange factor (GEF) 11
别名 241427A4, GTRAP48, KIAA0380, PDZ RHOGEF
计算分子量168 kDa
基因名称 ARHGEF11
Gene ID (NCBI) 9826
RRIDAB_3671713
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15085
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGEF11 (Rho Guanine Nucleotide Exchange Factor 11), also known as PDZ-RhoGEF or GTRAP48. ARHGEF11 contains several functional domains, including a PDZ domain and a RhoGEF domain. The PDZ domain allows it to interact with various proteins, such as ZO-1 and plexins, while the RhoGEF domain enables it to activate Rho GTPases. ARHGEF11 is a multifunctional protein that plays crucial roles in cell adhesion, signal transduction, and development. Its dysregulation has been linked to several diseases, making it an important area of research for potential therapeutic applications.

实验方案

Product Specific Protocols
IHC protocol for ARHGEF11 antibody 84149-1-RRDownload protocol
IF protocol for ARHGEF11 antibody 84149-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols