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验证数据展示

  • IHC staining of mouse colon using 30508-1-AP

    IHC staining of mouse colon using 30508-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30508-1-AP (ARHGEF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30508-1-AP

    WB analysis using 30508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30508-1-AP (ARHGEF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ARHGEF11 Polyclonal antibody

ARHGEF11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30508-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

ARHGEF11, DKFZp667F1223, GTRAP48, KIAA0380, PDZ RHOGEF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HuH-7 cells, MDA-MB-453 cells, mouse brain tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30508-1-AP targets ARHGEF11 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 ARHGEF11 fusion protein Ag33104 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho guanine nucleotide exchange factor (GEF) 11
别名 ARHGEF11, DKFZp667F1223, GTRAP48, KIAA0380, PDZ RHOGEF
计算分子量168 kDa
观测分子量200-220 kDa
基因名称 ARHGEF11
Gene ID (NCBI) 9826
RRIDAB_3086341
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15085
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).

实验方案

Product Specific Protocols
WB protocol for ARHGEF11 antibody 30508-1-APDownload protocol
IHC protocol for ARHGEF11 antibody 30508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols