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ARHGEF1 Polyclonal antibody

ARHGEF1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11363-1-AP

Print datasheet

Synonyms

ARHGEF1, GEF1, LBCL2, LSC, P115 RHOGEF, p115RhoGEF, SUB1.5

验证数据展示

  • WB analysis of HEK-293 using 11363-1-AP

    WB analysis of HEK-293 using 11363-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 11363-1-AP

    IF Staining of HeLa using 11363-1-AP

    Immunofluorescent analysis of HeLa cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 11363-1-AP

    IHC staining of human colon using 11363-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 11363-1-AP

    IHC staining of human colon using 11363-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HEK-293 using 11363-1-AP

    IP experiment of HEK-293 using 11363-1-AP

    IP result of anti-ARHGEF1 (IP:11363-1-AP, 4ug; Detection:11363-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, human spleen tissue, Jurkat cells, K-562 cells, mouse spleen tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, A549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11363-1-AP targets ARHGEF1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ARHGEF1 fusion protein Ag1905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho guanine nucleotide exchange factor (GEF) 1
Synonyms ARHGEF1, GEF1, LBCL2, LSC, P115 RHOGEF, p115RhoGEF, SUB1.5
Calculated Molecular Weight 912 aa, 102 kDa
Observed Molecular Weight 102-110 kDa
GenBank Accession NumberBC011726
Gene Symbol ARHGEF1
Gene ID (NCBI) 9138
RRIDAB_2059741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92888
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGEF1 antibody 11363-1-APDownload protocol
IHC protocol for ARHGEF1 antibody 11363-1-APDownload protocol
IF protocol for ARHGEF1 antibody 11363-1-APDownload protocol
IP protocol for ARHGEF1 antibody 11363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.

Authors - Silvana Konermann
View Article
humanWB

Nat Metab

Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression

Authors - Lin Qian
View Article
humanWB

Mol Biol Cell

The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.

Authors - P V Ryder
View Article
mouseCoIP,WB

Nat Commun

Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis

Authors - Yaping Zhang
View Article