验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HL-60 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
15665-1-AP targets ARHGAP9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag8104 Product name: Recombinant human ARHGAP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 402-750 aa of BC006107 Sequence: VLHIRTIPGHEFLLQSDHETELRAWHRALRTVIERLVRWVEARREAPTGRDQGSGDRENPLELRLSGSGPAELSAGEDEEEESELVSKPLLRLSSRRSSIRGPEGTEQNRVRNKLKRLIAKRPPLQSLQERGLLRDQVFGCQLESLCQREGDTVPSFLRLCIAAVDKRGLDVDGIYRVSGNLAVVQKLRFLVDRERAVTSDGRYVFPEQPGQEGRLDLDSTEWDDIHVVTGALKLFLRELPQPLVPPLLLPHFRAALALSESEQCLSQIQELIGSMPKPNHDTLRYLLEHLCRVIAHSDKNRMTPHNLGIVFGPTLFRPEQETSDPAAHALYPGQLVQLMLTNFTSLFP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho GTPase activating protein 9 |
| 别名 | Rho-type GTPase-activating protein 9, Rho GTPase-activating protein 9, RGL1, 10C |
| 计算分子量 | 750 aa, 83 kDa |
| 观测分子量 | 70 kDa, 65 kDa |
| GenBank蛋白编号 | BC006107 |
| 基因名称 | ARHGAP9 |
| Gene ID (NCBI) | 64333 |
| RRID | AB_2059566 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRR9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARHGAP9 belongs to RHO family of small GTPases, which inactivate RHO GTPases by stimulating their intrinsic GTPase activity. Several ARHGAP9 transcript isoforms were reported: including calculated mass of 81-83 kDa, 71 kDa and 61-63 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ARHGAP9 antibody 15665-1-AP | Download protocol |
| WB protocol for ARHGAP9 antibody 15665-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
