验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HeLa cells, HL-60 cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
15665-1-AP targets ARHGAP9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | ARHGAP9 fusion protein Ag8104 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Rho GTPase activating protein 9 |
别名 | Rho-type GTPase-activating protein 9, Rho GTPase-activating protein 9, RGL1, 10C |
计算分子量 | 750 aa, 83 kDa |
观测分子量 | 70 kDa, 65 kDa |
GenBank蛋白编号 | BC006107 |
基因名称 | ARHGAP9 |
Gene ID (NCBI) | 64333 |
RRID | AB_2059566 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BRR9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARHGAP9 belongs to RHO family of small GTPases, which inactivate RHO GTPases by stimulating their intrinsic GTPase activity. Several ARHGAP9 transcript isoforms were reported: including calculated mass of 81-83 kDa, 71 kDa and 61-63 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for ARHGAP9 antibody 15665-1-AP | Download protocol |
IF protocol for ARHGAP9 antibody 15665-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |