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ARHGAP27 Polyclonal antibody

ARHGAP27 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 22077-1-AP

Print datasheet

Synonyms

ARHGAP27, CAMGAP1, SH3D20

验证数据展示

  • WB analysis of A549 using 22077-1-AP

    WB analysis of A549 using 22077-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22077-1-AP (ARHGAP27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22077-1-AP

    IHC staining of human breast cancer using 22077-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 22077-1-AP

    IHC staining of human breast cancer using 22077-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 22077-1-AP

    IF Staining of HeLa using 22077-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22077-1-AP is ARHGAP27 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human
Immunogen ARHGAP27 fusion protein Ag17225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase activating protein 27
Synonyms ARHGAP27, CAMGAP1, SH3D20
Calculated Molecular Weight 548 aa, 62 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC101388
Gene Symbol ARHGAP27
Gene ID (NCBI) 201176
RRIDAB_2878985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZUM4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGAP27 antibody 22077-1-APDownload protocol
IHC protocol for ARHGAP27 antibody 22077-1-APDownload protocol
IF protocol for ARHGAP27 antibody 22077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols