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GRAF Polyclonal antibody

GRAF Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17747-1-AP

Print datasheet

Synonyms

ARHGAP26, GRAF, KIAA0621, Oligophrenin 1 like protein, OPHN1L, OPHN1L1

验证数据展示

  • WB analysis of HeLa using 17747-1-AP

    WB analysis of HeLa using 17747-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 17747-1-AP

    IP experiment of HepG2 using 17747-1-AP

    IP result of anti-GRAF (IP:17747-1-AP, 4ug; Detection:17747-1-AP 1:1000) with HepG2 cells lysate 3600ug.

  • IHC staining of human liver using 17747-1-AP

    IHC staining of human liver using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 17747-1-AP

    IHC staining of human liver using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, mouse thymus tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17747-1-AP targets GRAF in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen GRAF fusion protein Ag12008 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase activating protein 26
Synonyms ARHGAP26, GRAF, KIAA0621, Oligophrenin 1 like protein, OPHN1L, OPHN1L1
Calculated Molecular Weight 759 aa, 86 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC068555
Gene Symbol ARHGAP26
Gene ID (NCBI) 23092
RRIDAB_2059284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRAF antibody 17747-1-APDownload protocol
IHC protocol for GRAF antibody 17747-1-APDownload protocol
IP protocol for GRAF antibody 17747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Neurol

Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis

Authors - Sven Jarius
View Article
humanWB

Brain Res

Deficiency of NEAT1 prevented MPP+-induced inflammatory response, oxidative stress and apoptosis in dopaminergic SK-N-SH neuroblastoma cells via miR-1277-5p/ARHGAP26 axis.

Authors - Shufang Zhou
View Article
humanWB,IHC,CoIP

Cell Death Dis

CEMIP, acting as a scaffold protein for bridging GRAF1 and MIB1, promotes colorectal cancer metastasis via activating CDC42/MAPK pathway

Authors - Guojie Xu
View Article