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验证数据展示

  • WB analysis using 14349-1-AP

    WB analysis using 14349-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14349-1-AP (ARHGAP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 14349-1-AP

    IP experiment of Jurkat using 14349-1-AP

    IP result of anti-ARHGAP25 (IP:14349-1-AP, 4ug; Detection:14349-1-AP 1:500) with Jurkat cells lysate 1000ug.

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of Jurkat using 14349-1-AP

    WB analysis of Jurkat using 14349-1-AP

    WB result of ARHGAP25 antibody (14349-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARHGAP25 transfected Jurkat cells.

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 14349-1-AP

    IF Staining of A549 using 14349-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ARHGAP25 antibody (14349-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

ARHGAP25 Polyclonal antibody

ARHGAP25 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14349-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

KIAA0053, Rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Ramos cells, THP-1 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14349-1-AP targets ARHGAP25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ARHGAP25 fusion protein Ag5691 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho GTPase activating protein 25
别名 KIAA0053, Rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25
计算分子量 73 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC039591
基因名称 ARHGAP25
Gene ID (NCBI) 9938
RRIDAB_2059273
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42331
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGAP25 (Rho GTPase-activating protein 25) also known as KAIA0053, belongs to a family of Rho GTPase-modulating proteins which are implicated in actin remodeling,cell polarity, and cell migration. ARHGAP25 has a key role in the pathomechanism of autoantibody-induced arthritis in which it regulates inflammation.

实验方案

Product Specific Protocols
WB protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IHC protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IF protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IP protocol for ARHGAP25 antibody 14349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Carcinogenesis

ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop

Authors - Sijia Han
  • KD Validated
View Article