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验证数据展示

  • WB analysis of A549 using 31938-1-AP

    WB analysis of A549 using 31938-1-AP

    WB result of ARHGAP23 antibody (31938-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARHGAP23 transfected A549 cells.

  • WB analysis using 31938-1-AP

    WB analysis using 31938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31938-1-AP (ARHGAP23 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 31938-1-AP

    IHC staining of mouse testis using 31938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31938-1-AP (ARHGAP23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGAP23 antibody included
  • IHC staining of mouse testis using 31938-1-AP

    IHC staining of mouse testis using 31938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31938-1-AP (ARHGAP23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGAP23 antibody included
  • KD/KO Validated

ARHGAP23 Polyclonal antibody

ARHGAP23 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31938-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

KIAA1501, Rho GTPase Activating Protein 23, Rho GTPase-activating protein 23, Rho-type GTPase-activating protein 23

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, Calu-1 cells, HeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® ARHGAP23 Ready-To-Use IHC Kit. ARHGAP23 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31938-1-AP targets ARHGAP23 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag36340

Product name: Recombinant human ARHGAP23 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 500-700 aa of NM_001199417

Sequence: KVQLTPARQMNLGFGDESPEPEASGRGERLGRKVAPLATTEDSLASIPFIDEPTSPSIDLQAKHVPASAVVSSAMNSAPVLGTSPSSPTFTFTLGRHYSQDCSSIKAGRRSSYLLAITTERSKSCDDGLNTFRDEGRVLRRLPNRIPSLRMLRSFFTDGSLDSWGTSEDADAPSKRHSTSDLSDATFSDIRREGWLYYKQI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho GTPase activating protein 23
别名 KIAA1501, Rho GTPase Activating Protein 23, Rho GTPase-activating protein 23, Rho-type GTPase-activating protein 23
计算分子量162kDa
观测分子量140-150 kDa
GenBank蛋白编号NM_001199417
基因名称 ARHGAP23
Gene ID (NCBI) 57636
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P227
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGAP23 (Rho GTPase Activating Protein 23) belongs to the family of Rho GTPase activating proteins. The protein functions as a GTPase-activating factor by converting Rho class GTPases into their inactive GDP-bound state. ARHGAP23 is involved in the Rho GTPase signaling pathway, affecting processes such as cytoskeletal reorganization, cell migration, and cell proliferation. In non-small cell lung cancer, the expression of ARHGAP23 is associated with tumor immune infiltration and prognosis, and its role in tumor development has attracted attention. Mutations in this gene are related to a variety of diseases, including certain types of cancer and neurological disorders.

实验方案

Product Specific Protocols
IHC protocol for ARHGAP23 antibody 31938-1-APDownload protocol
WB protocol for ARHGAP23 antibody 31938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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