验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, Jurkat cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11940-1-AP targets ARHGAP15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2538 Product name: Recombinant human ARHGAP15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 176-475 aa of BC016701 Sequence: IILDWFHAIKNAIDRLPKDSSCPSRNLELFKIQRSSSTELLSHYDSDIKEQKPEHRKSLMFRLHHSASDTSDKNRVKSRLKKFITRRPSLKTLQEKGLIKDQIFGSHLHKVCERENSTVPWFVKQCIEAVEKRGLDVDGIYRVSGNLATIQKLRFIVNQEEKLNLDDSQWEDIHVVTGALKMFFRELPEPLFPYSFFEQFVEAIKKQDNNTRIEAVKSLVQKLPPPNRDTMKVPFGHLTKIVAKASKNLMSTQSLGIVFGPTLLRAENETGNMAIHMVYQNQIAELMLSEYSKIFGSEED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho GTPase activating protein 15 |
| 别名 | BM-046, BM046, BM-030, BM-024, BM 046 |
| 计算分子量 | 475 aa, 55 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC016701 |
| 基因名称 | ARHGAP15 |
| Gene ID (NCBI) | 55843 |
| RRID | AB_874081 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53QZ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARHGAP15 (Rho GTPase Activating Protein 15) is a Rac1-specific GAP. Diseases associated with ARHGAP15 include Diverticulitis and Meier-Gorlin Syndrome 2. Among its related pathways are Signaling by Rho GTPases and RAC2 GTPase cycle. ARHGAP15 deregulation is implicated in many abnormalities (PMID: 29867200). ARHGAP15 decreased in glioma, which promoted the aggressive phenotypes of glioma cells through the activation of Rac19. Intronic mutation of ARHGAP15 is associated with diverticular disease. In addition, ARHGAP15 was a prognosis-related biomarker for pancreatic ductal adenocarcinoma (PMID: 28979141).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ARHGAP15 antibody 11940-1-AP | Download protocol |
| IHC protocol for ARHGAP15 antibody 11940-1-AP | Download protocol |
| WB protocol for ARHGAP15 antibody 11940-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area. | ||
Cancer Sci Forkhead box P3 regulates ARHGAP15 expression and affects migration of glioma cells through the Rac1 signaling pathway.
| ||
J Neurosci Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation. |
