验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, SK-N-SH cells, U-251 cells, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16746-1-AP targets ARHGAP11B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10155 Product name: Recombinant human ARHGAP11B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-267 aa of BC105788 Sequence: MWDQRLVKLALLQHLRAFYGIKVKGVRGQCDRRRHETAATEIGGKIFGVPFNALPHSAVPEYGHIPSFLVDACTSLEEHIHTEGLFRKSGSVIRLKALKNKVDHGEGCLSSAPPCDIAGLLKQFFRELPEPILPADLHEALLKAQQLGTEEKNKAILLLSCLLADHTVHVLRYFFNFLRNVSLRSSENKMDSSNLAVIFAPNLLQTSEGHEKMSSNAEKKGVYQTLSWKRYQPCWVLMVSVLLHHWKALKKVNMKLLVNIREREDNV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho GTPase activating protein 11B |
| 别名 | FAM7B1, Inactive Rho GTPase-activating protein 11B, Rho-type GTPase-activating protein 11B |
| 计算分子量 | 267 aa, 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC105788 |
| 基因名称 | ARHGAP11B |
| Gene ID (NCBI) | 89839 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3KRB8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARHGAP11B is a hominin-specific protein that promotes the development and evolutionary expansion of the brain neocortex(PMID: 33938018). ARHGAP11B arose from partial duplication of ARHGAP11A on the human lineage after separation from the chimpanzee lineage, but before the divergence from Neandertals. The antibody may cross-react with the high homology N-terminal domain of the rodent Arhgap11a, specifically with its short splicing variants, mimicking the human protein size.(PMID: 27957544)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGAP11B antibody 16746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
