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验证数据展示

  • WB analysis using 66932-2-Ig

    WB analysis using 66932-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66932-2-Ig (ARH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of HeLa using 66932-2-Ig

    FC experiment of HeLa using 66932-2-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug ARH Monoclonal antibody (66932-2-Ig, Clone:1D1B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ARH Monoclonal antibody

ARH Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66932-2-Ig

产品说明书

CloneNo. 1D1B11
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

LDLRAP1, FHCB2, FHCB1, ARH2, ARH1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, THP-1 cells, HSC-T6 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66932-2-Ig targets ARH in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原

CatNo: Ag28608

Product name: Recombinant human LDLRAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-308 aa of BC029770

Sequence: MDALKSAGRALIRSPSLAKQSWGGGGRHRKLPENWTDTRETLLEGMLFSLKYLGMTLVEQPKGEELSAAAIKRIVATAKASGKKLQKVTLKVSPRGIILTDNLTNQLIENVSIYRISYCTADKMHDKVFAYIAQSQHNQSLECHAFLCTKRKMAQAVTLTVAQAFKVAFEFWQVSKEEKEKRDKASQEGGDVLGARQDCTPPLKSLVATGNLLDLEETAKAPLSTVSANTTNMDEVPRPQALSGSSVVWELDDGLDEAFSRLAQSRTNPQVLDTGLTAQDMHYAQCLSPVDWDKPDSSGTEQDDLFSF

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 low density lipoprotein receptor adaptor protein 1
别名 LDLRAP1, FHCB2, FHCB1, ARH2, ARH1
计算分子量 308 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC029770
基因名称 ARH
Gene ID (NCBI) 26119
RRIDAB_2882258
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5SW96
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARH antibody 66932-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Leukocyte immunoglobulin-like receptor B1 (LILRB1) protects human multiple myeloma cells from ferroptosis by maintaining cholesterol homeostasis

Authors - Miao Xian
  • KD Validated
View Article