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验证数据展示

  • WB analysis of HeLa using 14548-1-AP

    WB analysis of HeLa using 14548-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14548-1-AP

    IF Staining of HeLa using 14548-1-AP

    Immunofluorescent analysis of Hela cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human liver using 14548-1-AP

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ARFIP2 antibody included
  • IHC staining of human liver using 14548-1-AP

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ARFIP2 antibody included

ARFIP2 Polyclonal antibody

ARFIP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14548-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

POR1, Arfaptin-2, Arfaptin 2, ADP-ribosylation factor-interacting protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, human heart tissue, human liver tissue, mouse pancreas tissue
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARFIP2 Ready-To-Use IHC Kit. ARFIP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14548-1-AP targets ARFIP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag6076

Product name: Recombinant human ARFIP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 138-341 aa of BC000392

Sequence: RKYESVLQLGRALTAHLYSLLQTQHALGDAFADLSQKSPELQEEFGYNAETQKLLCKNGETLLGAVNFFVSSINTLVTKTMEDTLMTVKQYEAARLEYDAYRTDLEELSLGPRDAGTRGRLESAQATFQAHRDKYEKLRGDVAIKLKFLEENKIKVMHKQLLLFHNAVSAYFAGNQKQLEQTLQQFNIKLRPPGAEKPSWLEEQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor interacting protein 2
别名 POR1, Arfaptin-2, Arfaptin 2, ADP-ribosylation factor-interacting protein 2
计算分子量 38 kDa
观测分子量 38 kDa, 45 kDa
GenBank蛋白编号BC000392
基因名称 ARFIP2
Gene ID (NCBI) 23647
RRIDAB_2242930
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53365
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARFIP2 antibody 14548-1-APDownload protocol
IHC protocol for ARFIP2 antibody 14548-1-APDownload protocol
IF protocol for ARFIP2 antibody 14548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

Deciphering the immunological and prognostic features of hepatocellular carcinoma through ADP-ribosylation-related genes analysis and identify potential therapeutic target ARFIP2

Authors - Fenfen Jiang
View Article
human,mouseIHC,WB,IF

J Hepatocell Carcinoma

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway

Authors - Kaida Huang
View Article