验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, Raw 264.7 cells, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ARFIP1 Ready-To-Use IHC Kit. ARFIP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17726-1-AP targets Arfaptin-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag12122 Product name: Recombinant human ARFIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-373 aa of BC103759 Sequence: MAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGSPAPPLPSVMSPSRVAASRLAQQGSDLIVPAGGQRTQTKSGPVILADEIKNPAMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKLKTPGVDAPSWLEEQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADP-ribosylation factor interacting protein 1 |
| 别名 | ARFIP1, ADP-ribosylation factor-interacting protein 1, Arfaptin 1 |
| 计算分子量 | 373 aa, 42 kDa |
| 观测分子量 | 42 kDa, 38 kDa, 22 kDa |
| GenBank蛋白编号 | BC103759 |
| 基因名称 | Arfaptin-1 |
| Gene ID (NCBI) | 27236 |
| RRID | AB_2227457 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P53367 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Arfaptin-1 antibody 17726-1-AP | Download protocol |
| IHC protocol for Arfaptin-1 antibody 17726-1-AP | Download protocol |
| WB protocol for Arfaptin-1 antibody 17726-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
