• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Arfaptin-1 Polyclonal antibody

Arfaptin-1 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 17726-1-AP

Print datasheet

Synonyms

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521

验证数据展示

  • WB analysis using 17726-1-AP

    WB analysis using 17726-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17726-1-AP (Arfaptin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17726-1-AP

    IHC staining of human breast cancer using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17726-1-AP (ARFIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IHC staining of human breast cancer using 17726-1-AP

    IHC staining of human breast cancer using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17726-1-AP (ARFIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IF Staining of HepG2 using 17726-1-AP

    IF Staining of HepG2 using 17726-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Arfaptin-1 antibody (17726-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inBxPC-3 cells, HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, Raw 264.7 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARFIP1 Ready-To-Use IHC Kit. ARFIP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17726-1-AP targets Arfaptin-1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Arfaptin-1 fusion protein Ag12122 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor interacting protein 1
Synonyms Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521
Calculated Molecular Weight 373 aa, 42 kDa
Observed Molecular Weight 42 kDa, 38 kDa, 22 kDa
GenBank Accession NumberBC103759
Gene Symbol ARFIP1
Gene ID (NCBI) 27236
RRIDAB_2227457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53367
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IHC protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IF protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Authors - Liyuan Zhang
View Article