验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, HepG2 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67279-1-Ig targets ARF5 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag7849 Product name: Recombinant human ARF5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-180 aa of BC003043 Sequence: MGLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ADP-ribosylation factor 5 |
| 别名 | ADP ribosylation factor 5, ARF5 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC003043 |
| 基因名称 | ARF5 |
| Gene ID (NCBI) | 381 |
| RRID | AB_2882547 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P84085 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARF5 antibody 67279-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
