Urine of six individual healthy human donors was measured. The Amphiregulin concentration of detected samples was determined to be 7.4 ng/mL with a range of 2.3-12.5 ng/mL.

Saliva of six individual healthy human donors was measured. The Amphiregulin concentration of detected samples was determined to be 95.9 ng/mL with a range of 22.9-168.9 ng/mL.

MDA-MB-453 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human Amphiregulin, and measured 915.6 pg/mL.

The mean Amphiregulin concentration was determined to be 378.9 ng/mL in MDA-MB-453 cell extract based on a 1.8 mg/mL extract load.

Plasma of eight individual healthy human donors was measured. The Amphiregulin concentration of detected samples was determined to be 15.22 pg/mL with a range of 6.47-20.52 pg/mL.

Sandwich ELISA standard curve of MP02406-2, Human Amphiregulin Recombinant Matched Antibody Pair - PBS only. 86387-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4569. 86387-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

MCF-7 human breast cancer cells were cultured stimulated with PMA for 3 days. An aliquot of the culture supernatant was removed, assayed for human Amphiregulin, and measured 96.4 ng/mL.

Cytometric bead array standard curve of MP02406-1, Amphiregulin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86387-2-PBS. Detection antibody: 86387-1-PBS. Standard: Eg4569. Range: 0.781-50 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 86387-1-PBS against Human AREG were performed. The affinity constant is 1.79 nM.