验证数据展示
经过测试的应用
| Positive IHC detected in | human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22578-1-AP targets ARAP3 in IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag17436 Product name: Recombinant human ARAP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1225-1544 aa of BC009968 Sequence: PRVGLLRCREEPPRLLGSRFQERFFLLRGRCLLLLKEKKSSKPEREWPLEGAKVYLGIRKKLKPPTPWGFTLILEKMHLYLSCTDEDEMWDWTTSILKAQHDDQQPVVLRRHSSSDLARQKFGTMPLLPIRGDDSGATLLSANQTLRRLHNRRTLSMFFPMKSSQGSVEEQEELEEPVYEEPVYEEVGAFPELIQDTSTSFSTTREWTVKPENPLTSQKSLDQPFLSKSSTLGQEERPPEPPPGPPSKSSPQARGSLEEQLLQELSSLILRKGETTAGLGSPSQPTSPQSPSPTGLPTQTPGFPTQPPCTSSPPSSQPLT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 |
| 别名 | ARAP3, Centaurin delta 3, CENTD3, Cnt d3, DRAG1 |
| 计算分子量 | 1544 aa, 170 kDa |
| GenBank蛋白编号 | BC009968 |
| 基因名称 | ARAP3 |
| Gene ID (NCBI) | 64411 |
| RRID | AB_2879125 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WWN8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ADP-ribosylation factor (ARF) family of small GTP-binding proteins are involved in vesicular transport regulation and in controlling cytoskeletal organization and cell adhesion. These proteins are best characterized as regulators of membrane traffic. The Centaurin GTPase-activating protein family comprise a subset of ARF regulatory molecules that transduce PI 3-kinase activation into coordinated control of ARF-dependent pathways. ARAP3 (Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3) is unique for its dual specificity GAPs (GTPase activating protein) activity for Arf6 (ADP-ribosylation factor 6) and RhoA (Ras homolog gene family member A) regulated by PtdIns(3,4,5)P3 (phosphatidylinositol (3,4,5)-trisphosphate) and a small GTPase Rap1-GTP and is involved in regulation of cell shape and adhesion.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARAP3 antibody 22578-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
