验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17541-1-AP targets ARAP1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag11597 Product name: Recombinant human ARAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 329-679 aa of BC056401 Sequence: LYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRMSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 |
| 别名 | Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1, Centaurin-delta-2, CENTD2, Cnt-d2, KIAA0782 |
| 计算分子量 | 162 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | BC056401 |
| 基因名称 | ARAP1 |
| Gene ID (NCBI) | 116985 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96P48 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARAP1 is a member of ARAP family with both Arf GAP and Rho GAP activities, by which ARAP1 makes a direct link between ADP-ribosylation factor (Arf) GTPases and Rho GTPases signaling. ARAP1 is involved in membrane trafficking and receptor recycling. It supports the plasma membrane recycling of the angiotensin II type 1 receptor (AT1) and regulates the cell-specific trafficking of the pro-death receptor TRAIL-R1/DR4, thereby promoting TRAIL-induced apoptosis in certain cell types. (PMID: 14559250, PMID: 18165900, PMID: 38008882)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARAP1 antibody 17541-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
