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验证数据展示

  • WB analysis of HEK-293 using 24342-1-AP

    WB analysis of HEK-293 using 24342-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 24342-1-AP

    IHC staining of human brain using 24342-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

  • IHC staining of human kidney using 24342-1-AP

    IHC staining of human kidney using 24342-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

  • IP experiment of HEK-293 using 24342-1-AP

    IP experiment of HEK-293 using 24342-1-AP

    IP result of anti-AQR (IP:24342-1-AP, 4ug; Detection:24342-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

  • IF Staining of U2OS using 24342-1-AP

    IF Staining of U2OS using 24342-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using AQR antibody (24342-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of U2OS using 24342-1-AP

    IF Staining of U2OS using 24342-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using AQR antibody (24342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

AQR Polyclonal antibody

AQR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 24342-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

KIAA0560, Intron-binding protein of 160 kDa, IBP160, fSAP164, EC:3.6.4.13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24342-1-AP targets AQR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 AQR fusion protein Ag17810 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aquarius homolog (mouse)
别名 KIAA0560, Intron-binding protein of 160 kDa, IBP160, fSAP164, EC:3.6.4.13
计算分子量 1485 aa, 171 kDa
观测分子量 160-171 kDa
GenBank蛋白编号BC127111
基因名称 AQR
Gene ID (NCBI) 9716
RRIDAB_2879501
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60306
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AQR antibody 24342-1-APDownload protocol
IHC protocol for AQR antibody 24342-1-APDownload protocol
IF protocol for AQR antibody 24342-1-APDownload protocol
IP protocol for AQR antibody 24342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Cell

Efficient Pre-mRNA Cleavage Prevents Replication-Stress-Associated Genome Instability.

Authors - Federico Teloni
View Article