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验证数据展示

  • IHC staining of human liver using 20380-1-AP

    IHC staining of human liver using 20380-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 20380-1-AP

    WB analysis using 20380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 20380-1-AP

    WB analysis using 20380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 20380-1-AP

    IF Staining of mouse brain using 20380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human liver using 20380-1-AP

    IF Staining of human liver using 20380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human liver using 20380-1-AP

    IF Staining of human liver using 20380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

AQP9 Polyclonal antibody

AQP9 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 20380-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (1)

别名

HsT17287, Aquaporin-9, Aquaglyceroporin-9, Aquaglyceroporin 9, AQP-9

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, mouse brain tissue, rat brain tissue, mouse liver tissue, mouse lung tissue, mouse testis tissue, rat liver tissue, rat testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue, mouse brain tissue, mouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20380-1-AP targets AQP9 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag14200

Product name: Recombinant human AQP9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 211-295 aa of BC026258

Sequence: SGCAMNPARDLSPRLFTALAGWGFEVFRAGNNFWWIPVVGPLVGAVIGGLIYVLVIEIHHPEPDSVFKAEQSEDKPEKYELSVIM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aquaporin 9
别名 HsT17287, Aquaporin-9, Aquaglyceroporin-9, Aquaglyceroporin 9, AQP-9
计算分子量 295 aa, 31 kDa
观测分子量31 kDa
GenBank蛋白编号BC026258
基因名称 AQP9
Gene ID (NCBI) 366
RRIDAB_2935448
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43315
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aquaporins (AQPs), also known as water channel proteins, are members of a large protein family that osmotically modulate water fluid homeostasis in several tissues. AQP9 (Aquaporin 9) is a member of the aquaporin family that acts as a water-selective membrane channel, and it may be involved in cell migration, angiogenesis, and tumor growth. AQP9 is involved in arsenic uptake in hepatocellular carcinoma cells, in which p38-MAPK may play a limited role. AQP9 was demonstrated to promote astrocytoma cell invasion and motility via the AKT pathway.

实验方案

Product Specific Protocols
IF protocol for AQP9 antibody 20380-1-APDownload protocol
IHC protocol for AQP9 antibody 20380-1-APDownload protocol
WB protocol for AQP9 antibody 20380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Int J Med Sci

Exploring the Shared Diagnostic Genes in IBD and Psoriasis through Bioinformatics and Experimental Assays

Authors - Lichun Han
View Article
humanWB,IHC,CoIP

Sci Rep

Aquaporin 9 downregulation in KRASG12V colorectal cancer and associated with increased proliferation and decreased apoptosis in cancer cells

Authors - Yankui Liu
  • KD Validated
View Article
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