验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse testis tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 3 publications below |
产品信息
25131-1-AP targets AQP7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse, sheep |
| 免疫原 |
CatNo: Ag17945 Product name: Recombinant human AQP7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 68-122 aa of BC062701 Sequence: LAAATIYSLFYTAILHFSGGQLMVTGPVATAGIFATYLPDHMTLWRGFLNEAWLT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aquaporin 7 |
| 别名 | AQP 7, AQP-7, AQP9, Aquaglyceroporin-7, aquaporin 7 |
| 计算分子量 | 342 aa, 37 kDa |
| 观测分子量 | 25-30 kDa, 40 kDa |
| GenBank蛋白编号 | BC062701 |
| 基因名称 | AQP7 |
| Gene ID (NCBI) | 364 |
| RRID | AB_2879913 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14520 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AQP7 is a member of the aquaporin family of water-selective membrane channels, localizes to the plasma membrane, and allows movement of water, glycerol and urea across cell membranes. AQP7 forms a channel that mediates water and glycerol transport across cell membranes at neutral pH. AQP7 is highly expressed in the adipose tissue(PMID: 9405233).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for AQP7 antibody 25131-1-AP | Download protocol |
| IP protocol for AQP7 antibody 25131-1-AP | Download protocol |
| WB protocol for AQP7 antibody 25131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology Loss of MYO5B Leads to Reductions in Na+ Absorption With Maintenance of CFTR-Dependent Cl- Secretion in Enterocytes. | ||
FASEB J Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia | ||
Animals (Basel) Rumen-Degradable Starch Improves Rumen Fermentation, Function, and Growth Performance by Altering Bacteria and Its Metabolome in Sheep Fed Alfalfa Hay or Silage | ||
Biomed Pharmacother Central administration of Dapagliflozin alleviates a hypothalamic neuroinflammatory signature and changing tubular lipid metabolism in type 2 diabetic nephropathy by upregulating MCPIP1 |
