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验证数据展示

  • WB analysis of mouse kidney using 81137-2-RR

    WB analysis of mouse kidney using 81137-2-RR

    Mouse kidney total extract lysate and membrane extract lysate were subjected to SDS PAGE followed by western blot with 81137-2-RR (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat kidney using 81137-2-RR

    WB analysis of rat kidney using 81137-2-RR

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 81137-2-RR (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 81137-2-RR

    IHC staining of human kidney using 81137-2-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 81137-2-RR (AQP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AQP1 antibody included
  • IHC staining of mouse kidney using 81137-2-RR

    IHC staining of mouse kidney using 81137-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 81137-2-RR (AQP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AQP1 antibody included
  • IHC staining of mouse kidney using 81137-2-RR

    IHC staining of mouse kidney using 81137-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 81137-2-RR (AQP1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AQP1 antibody included
  • IF Staining of mouse kidney using 81137-2-RR

    IF Staining of mouse kidney using 81137-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using AQP1 antibody (81137-2-RR, Clone: 251798B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81137-2-RR

    Affinity and Kinetic Characterization of 81137-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81137-2-RR against Human AQP1 were performed. The affinity constant is 6.13 nM.

AQP1 Recombinant monoclonal antibody

AQP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 81137-2-RR

产品说明书

CloneNo. 251798B1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Aquaporin 1, AQP 1, AQP-1, Aquaporin1, Aquaporin-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy AQP1 Ready-To-Use IHC Kit. AQP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81137-2-RR targets AQP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14093

Product name: Recombinant human AQP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 220-269 aa of BC022486

Sequence: GALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aquaporin 1 (Colton blood group)
别名 Aquaporin 1, AQP 1, AQP-1, Aquaporin1, Aquaporin-1
计算分子量 269 aa, 29 kDa
观测分子量 25-28 kDa, 35-50 kDa
GenBank蛋白编号BC022486
基因名称 AQP1
Gene ID (NCBI) 358
RRIDAB_3743368
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP29972
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 35-50 kDa. (PMID:20965731,16508653, 1530176).

实验方案

Product Specific Protocols
IF protocol for AQP1 antibody 81137-2-RRDownload protocol
IHC protocol for AQP1 antibody 81137-2-RRDownload protocol
WB protocol for AQP1 antibody 81137-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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