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APPL1 Polyclonal antibody

APPL1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 19885-1-AP

Print datasheet

Synonyms

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428

验证数据展示

  • WB analysis of human brain using 19885-1-AP

    WB analysis of human brain using 19885-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 19885-1-AP

    IHC staining of human breast cancer using 19885-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 19885-1-AP (APPL1 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with APPL1 antibody included
  • IF Staining of HepG2 using 19885-1-AP

    IF Staining of HepG2 using 19885-1-AP

    Immunofluorescent analysis of HepG2 cells, using APPL1 antibody 19885-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse brain using 19885-1-AP

    IP experiment of mouse brain using 19885-1-AP

    IP result of anti-APPL1 (IP:19885-1-AP, 4ug; Detection:19885-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

  • WB analysis of HEK-293 using 19885-1-AP

    WB analysis of HEK-293 using 19885-1-AP

    WB result of APPL1 antibody (19885-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.

  • IF Staining of HepG2 using 19885-1-AP

    IF Staining of HepG2 using 19885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HepG2 using 19885-1-AP

    FC experiment of HepG2 using 19885-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (19885-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, HeLa cells, human heart tissue, HT-1080 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APPL1 Ready-To-Use IHC Kit. APPL1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19885-1-AP targets APPL1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen APPL1 fusion protein Ag13703 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Synonyms APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428
Calculated Molecular Weight 709 aa, 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC028599
Gene Symbol APPL1
Gene ID (NCBI) 26060
RRIDAB_10637848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKG1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

实验方案

Product Specific Protocols
WB protocol for APPL1 antibody 19885-1-APDownload protocol
IHC protocol for APPL1 antibody 19885-1-APDownload protocol
IF protocol for APPL1 antibody 19885-1-APDownload protocol
IP protocol for APPL1 antibody 19885-1-APDownload protocol
FC protocol for APPL1 antibody 19885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols