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验证数据展示

  • WB analysis using 82871-1-RR (same clone as 82871-1-PBS)

    WB analysis using 82871-1-RR (same clone as 82871-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82871-1-RR (APOM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82871-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82871-1-RR (same clone as 82871-1-PBS)

    IF Staining of HepG2 using 82871-1-RR (same clone as 82871-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (82871-1-RR, Clone: 3O19 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82871-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82871-1-RR (same clone as 82871-1-PBS)

    FC experiment of HepG2 using 82871-1-RR (same clone as 82871-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug APOM Recombinant antibody (82871-1-RR, Clone:3O19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82871-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82871-1-RR

    Affinity and Kinetic Characterization of 82871-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82871-1-RR against Human APOM were performed. The affinity constant is 0.694 nM.

APOM Recombinant monoclonal antibody, PBS Only

APOM Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82871-1-PBS

产品说明书

CloneNo. 3O19

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

3O19, Apo M, Apolipoprotein M, Apo-M, G3A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82871-1-PBS targets APOM in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3048

Product name: Recombinant human APOM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-188 aa of BC020683

Sequence: MFHQIWAALLYFYGIILNSIYQCPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein M
别名 3O19, Apo M, Apolipoprotein M, Apo-M, G3A
计算分子量 21 kDa
观测分子量 21-25 kDa
GenBank蛋白编号BC020683
基因名称 APOM
Gene ID (NCBI) 55937
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95445
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).

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