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APOL4 Polyclonal antibody

APOL4 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10564-1-AP

Print datasheet

Synonyms

APOL IV, APOL4, Apolipoprotein L IV, apolipoprotein L, 4, Apolipoprotein L4, APOLIV

验证数据展示

  • WB analysis using 10564-1-AP

    WB analysis using 10564-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10564-1-AP (APOL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 10564-1-AP

    IP experiment of HepG2 using 10564-1-AP

    IP result of anti-APOL4 (IP:10564-1-AP, 4ug; Detection:10564-1-AP 1:2000) with HepG2 cells lysate 4000ug.

  • IHC staining of human liver cancer using 10564-1-AP

    IHC staining of human liver cancer using 10564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 10564-1-AP

    IHC staining of human liver cancer using 10564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 10564-1-AP

    IF Staining of HepG2 using 10564-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10564-1-AP (APOL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, U-87 MG cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10564-1-AP targets APOL4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human
Immunogen APOL4 fusion protein Ag0849 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein L, 4
Synonyms APOL IV, APOL4, Apolipoprotein L IV, apolipoprotein L, 4, Apolipoprotein L4, APOLIV
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC006276
Gene Symbol APOL4
Gene ID (NCBI) 80832
RRIDAB_2242901
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPW4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APOL4 antibody 10564-1-APDownload protocol
IHC protocol for APOL4 antibody 10564-1-APDownload protocol
IF protocol for APOL4 antibody 10564-1-APDownload protocol
IP protocol for APOL4 antibody 10564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols