验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, BxPC-3 cells, HCT 116 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28591-1-AP targets APOL3 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag29687 Product name: Recombinant human APOL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 246-380 aa of NM_145640 Sequence: SSAEAEASRLTATSIDRLKVFKEVMRDITPNLLSLLNNYYEATQTIGSEIRAIRQARARARLPVTTWRISAGSGGQAERTIAGTTRAVSRGARILSATTSGIFLALDVVNLVYESKHLHEGAKSASAEELRRQAQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | apolipoprotein L, 3 |
| 别名 | ApoL III, APOL3, APOLIII, Apolipoprotein L III, apolipoprotein L, 3, Apolipoprotein L3, CG12 1, CG12_1, TNF inducible protein CG12 1 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | NM_145640 |
| 基因名称 | APOL3 |
| Gene ID (NCBI) | 80833 |
| RRID | AB_3669663 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95236 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apolipoprotein L3(APOL3) is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 22. APOL3 is localized in the cytoplasm and plays a central role in cholesterol transport, affecting the movement of lipids, including cholesterol, and allowing the binding of lipids to organelles.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APOL3 antibody 28591-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
