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  • Featured Product
  • KD/KO Validated

APOL2 Polyclonal antibody

APOL2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25925-1-AP

Print datasheet

Synonyms

APOL II, APOL2, APOL3, Apolipoprotein L II, apolipoprotein L, 2, Apolipoprotein L2

验证数据展示

  • WB analysis of A549 using 25925-1-AP

    WB analysis of A549 using 25925-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 25925-1-AP

    IHC staining of human lung cancer using 25925-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25925-1-AP (APOL2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human lung cancer using 25925-1-AP

    IHC staining of human lung cancer using 25925-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25925-1-AP (APOL2 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 25925-1-AP

    IF Staining of HeLa using 25925-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 25925-1-AP (APOL2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, A431 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25925-1-AP is APOL2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen APOL2 fusion protein Ag7622 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein L, 2
Synonyms APOL II, APOL2, APOL3, Apolipoprotein L II, apolipoprotein L, 2, Apolipoprotein L2
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC004395
Gene Symbol APOL2
Gene ID (NCBI) 23780
RRIDAB_2880297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BQE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APOL2 antibody 25925-1-APDownload protocol
IHC protocol for APOL2 antibody 25925-1-APDownload protocol
IF protocol for APOL2 antibody 25925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Chem Commun (Camb)

Competitive affinity-based proteome profiling and imaging to reveal potential cellular targets of betulinic acid.

Authors - Haijun Guo
  • KD Validated
View Article
humanWB

Aging (Albany NY)

TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs)

Authors - Jianyun Liu
View Article