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验证数据展示

  • WB analysis of human plasma using 16608-1-AP (same clone as 16608-1-PBS)

    WB analysis of human plasma using 16608-1-AP (same clone as 16608-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 16608-1-AP (APOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16608-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16608-1-AP (same clone as 16608-1-PBS)

    IHC staining of human liver using 16608-1-AP (same clone as 16608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16608-1-AP (APOF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16608-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16608-1-AP (same clone as 16608-1-PBS)

    IHC staining of human liver using 16608-1-AP (same clone as 16608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16608-1-AP (APOF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16608-1-PBS in a different storage buffer formulation.

APOF Polyclonal antibody, PBS Only

APOF Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16608-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Apo F, Apo-F, apolipoprotein F, LTIP

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16608-1-PBS targets APOF in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9824

Product name: Recombinant human APOF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 37-326 aa of BC026257

Sequence: TSYGKQTNVLMHFPLSLESQTPSSDPLSCQFLHPKSLPGFSHMAPLPKFLVSLALRNALEEAGCQADVWALQLQLYRQGGVNATQVLIQHLRGLQKGRSTERNVSVEALASALQLLAREQQSTGRVGRSLPTEDCENEKEQAVHNVVQLLPGVGTFYNLGTALYYATQNCLGKARERGRDGAIDLGYDLLMTMAGMSGGPMGLAISAALKPALRSGVQQLIQYYQDQKDANISQPETTKEGLRAISDVSDLEETTTLASFISEVVSSAPYWGWAIIKSYDLDPGAGSLEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein F
别名 Apo F, Apo-F, apolipoprotein F, LTIP
计算分子量 326 aa, 35 kDa
观测分子量 30-33 kDa
GenBank蛋白编号BC026257
基因名称 APOF
Gene ID (NCBI) 319
RRIDAB_2058253
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13790
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APOF protein, full name Apolipoprotein F, also known as Lipid transfer inhibitor protein (LTIP), belongs to the apolipoprotein F family. This protein is primarily expressed by the liver and secreted into the plasma. Its core function is to act as a cholesterol transport regulator, modulating the transport of cholesterol among plasma lipoproteins by inhibiting the activity of cholesteryl ester transfer protein (CETP). APOF is initially synthesized as a precursor protein with a theoretical molecular weight of approximately 35.4 kDa. After processing, the mature peptide has a molecular weight of about 17.4 kDa. However, in plasma, due to modifications such as glycosylation, the apparent molecular weight detected experimentally is typically around 33-35 kDa.

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