• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • IHC staining of mouse liver using 84775-7-RR

    IHC staining of mouse liver using 84775-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84775-7-RR

    IHC staining of mouse liver using 84775-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 84775-7-RR

    IF Staining of HepG2 using 84775-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (84775-7-RR, Clone: 242310B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84775-7-RR

    Affinity and Kinetic Characterization of 84775-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84775-7-RR against Human APOE were performed. The affinity constant is below 1 pM.

APOE Recombinant monoclonal antibody

APOE Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84775-7-RR

产品说明书

CloneNo. 242310B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84775-7-RR targets APOE in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Apolipoprotein E
别名 ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E
计算分子量 36 kDa
GenBank蛋白编号BC003557
基因名称 APOE
Gene ID (NCBI) 348
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP02649
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight (PMID: 9831633).

实验方案

Product Specific Protocols
IF protocol for APOE antibody 84775-7-RRDownload protocol
IHC protocol for APOE antibody 84775-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...