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验证数据展示

  • IHC staining of mouse liver using 84775-7-RR (same clone as 84775-7-PBS)

    IHC staining of mouse liver using 84775-7-RR (same clone as 84775-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84775-7-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84775-7-RR (same clone as 84775-7-PBS)

    IHC staining of mouse liver using 84775-7-RR (same clone as 84775-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84775-7-RR (APOE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84775-7-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84775-7-RR (same clone as 84775-7-PBS)

    IF Staining of HepG2 using 84775-7-RR (same clone as 84775-7-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (84775-7-RR, Clone: 242310B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84775-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84775-7-RR

    Affinity and Kinetic Characterization of 84775-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84775-7-RR against Human APOE were performed. The affinity constant is below 1 pM.

APOE Recombinant antibody, PBS Only

APOE Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 84775-7-PBS

产品说明书

CloneNo. 242310B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84775-7-PBS targets APOE in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Apolipoprotein E
别名 ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E
计算分子量 36 kDa
GenBank蛋白编号BC003557
基因名称 APOE
Gene ID (NCBI) 348
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP02649
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight (PMID: 9831633).