验证数据展示
经过测试的应用
| Positive WB detected in | human serum tissue, HepG2 cells, human plasma |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:800-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 4 publications below |
产品信息
10520-1-AP targets APOD in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0812 Product name: Recombinant human APOD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 15-189 aa of BC007402 Sequence: FGAAEGQAFHLGKCPNPPVQENFDVNKYLGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYWILATDYENYALVYSCTCIIQLFHVDFAWILARNPNLPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | apolipoprotein D |
| 别名 | Apo D, Apo-D, apolipoprotein D |
| 计算分子量 | 33 kDa |
| 观测分子量 | 21-33 kDa |
| GenBank蛋白编号 | BC007402 |
| 基因名称 | APOD |
| Gene ID (NCBI) | 347 |
| RRID | AB_2057977 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05090 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for APOD antibody 10520-1-AP | Download protocol |
| IHC protocol for APOD antibody 10520-1-AP | Download protocol |
| WB protocol for APOD antibody 10520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuropathol Appl Neurobiol Apolipoprotein D: a potential biomarker for cerebral amyloid angiopathy. | ||
Clin Proteomics Integration of transcriptome and proteome profiles in placenta accreta reveals trophoblast over-migration as the underlying pathogenesis.
| ||
Biomarkers Apolipoprotein D as a novel marker in human end-stage heart failure: a preliminary study. | ||
Front Immunol A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma | ||
Comput Struct Biotechnol J Uncovering the potential of APOD as a biomarker in gastric cancer: A retrospective and multi-center study |
