验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, HEK-293 cells, K-562 cells, SW480 cells |
| Positive IHC detected in | human ovary cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10591-1-AP targets APOBEC3C in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0886 Product name: Recombinant human APOBEC3C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC011739 Sequence: MNPQIRNPMKAMYPGTFYFQFKNLWEANDRDETWLCFTVEGIKRRSVVSWKTGVFRNQVDSETHCHAERCFLSWFCDDILSPNTKYQVTWYTSWSPCPDCAGEVAEFLARHSNVNLTIFTARLYYFQYPCYQEGLRSLSQEGVAVEIMDYEDFKYCWENFVYNDNEPFKPWKGLKTNFRLLKRRLRESLQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C |
| 别名 | ARDC2, APOBEC3, APOBEC1-like, APOBEC1L, APOBEC |
| 计算分子量 | 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC011739 |
| 基因名称 | APOBEC3C |
| Gene ID (NCBI) | 27350 |
| RRID | AB_2057393 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NRW3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
APOBEC3C(apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C) is also named as APOBEC1L, PBI and belongs to the cytidine and deoxycytidylate deaminase family.APOBEC3C is related to APOBEC1, a cytidine deaminase responsible for cytidine-to-uridine editing of apolipoprotein B (APOB)mRNA.It can form a dimer(PMID:19074429).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for APOBEC3C antibody 10591-1-AP | Download protocol |
| WB protocol for APOBEC3C antibody 10591-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Loss of SYNCRIP unleashes APOBEC-driven mutagenesis, tumor heterogeneity, and AR-targeted therapy resistance in prostate cancer | ||
Nat Commun A scalable two-step genome editing strategy for generating full-length gene-humanized mice at diverse genomic loci. | ||
Nucleic Acids Res Enterovirus 71 antagonizes the inhibition of the host intrinsic antiviral factor A3G. | ||
Cell Death Dis APOBEC3C coordinates DDX5 in R-loop resolution and dynamic control of Chk1-mediated stress-responsive circuitry as a prerequisite for gemcitabine resistance in p53-deficient cells.
| ||
Int J Biol Macromol Pan-cancer analysis and experimental validation revealed that prognostic biomarker APOBEC3C may promote M2-type macrophage polarization and prevent R-loop accumulation in glioma.
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