验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25328-1-AP targets APOB48R in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18204 Product name: Recombinant human APOB48R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 851-1088 aa of BC119786 Sequence: SQTARAEGMGAMVEAGGLLEKWTLLEEEAVGWQEREQREDSEGRCGDYHPEGEAPRLLDAEGLMVTGGRRAEAKETEPESLEHVRGQEEQPTHQAPAEAAPESVGEAETAEAMGSARGGAANSWSEAPLPGSLLDVSVPRSRVHLSRSSSQRRSRPSFRRTPAWEQQEEPPAPNPPEEELSAPEQRPLQLEEPLEPSPLRHDGTPVPARRRPLGHGFGLAHPGMMQELQARLGRPKPQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | apolipoprotein B48 receptor |
| 别名 | apoB 48R, apoB-48R, APOBR, Apolipoprotein B 100 receptor, Apolipoprotein B 48 receptor |
| 计算分子量 | 1088 aa, 116 kDa |
| 观测分子量 | 116 kDa |
| GenBank蛋白编号 | BC119786 |
| 基因名称 | APOB48R |
| Gene ID (NCBI) | 55911 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q0VD83 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apolipoprotein B48 receptor (APOB48R) is a macrophage receptor that binds to apoB of dietary triglyceride-rich lipoproteins or to a similar apoB-like domain in hypertriglyceridemic very low-density lipoprotein (HTG-VLDL). This receptor is crucial for the uptake of chylomicrons, HTG-VLDL, and trypsinized VLDL lacking apoE in macrophages. APOB48R may contribute necessary lipids, lipid-soluble vitamins, and other nutrients to reticuloendothelial cells. Elevated plasma triglyceride levels may cause APOB48R to be a factor in foam cell formation, endothelial dysfunction, and atherothrombogenesis (PMID: 10852956; 9633939; 15591219).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APOB48R antibody 25328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
