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验证数据展示

  • WB analysis using RMX00011

    WB analysis using RMX00011

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00011 (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using RMX00011

    IHC staining of mouse liver using RMX00011

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00011 (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using RMX00011

    IF Staining of mouse liver using RMX00011

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using APOB antibody (RMX00011) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Multi-rAb™ APOB Multi-Recombinant antibody

APOB Multi-Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. RMX00011

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

Apolipoprotein B, Apolipoprotein B-100, ApoB48, ApoB100, Apo B-100

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00011 targets APOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 apolipoprotein B (including Ag(x) antigen)
别名 Apolipoprotein B, Apolipoprotein B-100, ApoB48, ApoB100, Apo B-100
计算分子量 516 kDa
观测分子量150-250 kDa, 400-520 kDa
GenBank蛋白编号NM_000384
基因名称 APOB
Gene ID (NCBI) 338
偶联类型 Unconjugated
形式 Liquid
纯化方式N/A
UNIPROT IDP04114
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).

实验方案

Product Specific Protocols
WB protocol for APOB antibody RMX00011Download protocol
IHC protocol for APOB antibody RMX00011Download protocol
IF protocol for APOB antibody RMX00011Download protocol
Standard Protocols
Click here to view our Standard Protocols