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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

    WB analysis of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 84047-1-RR (APOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84047-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

    IHC staining of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84047-1-RR (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84047-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

    IF Staining of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using APOB antibody (84047-1-RR, Clone: 240884H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84047-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

    IF Staining of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using APOB antibody (84047-1-RR, Clone: 240884H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84047-1-PBS in a different storage buffer formulation.

APOB Recombinant antibody, PBS Only

APOB Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84047-1-PBS

产品说明书

CloneNo. 240884H4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

Apolipoprotein B, ApoB48, ApoB100, Apo B-100, Apo B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84047-1-PBS targets APOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein B (including Ag(x) antigen)
别名 Apolipoprotein B, ApoB48, ApoB100, Apo B-100, Apo B
计算分子量 516 kDa
观测分子量150-250 kDa, 400-520 kDa
GenBank蛋白编号NM_000384
基因名称 APOB
Gene ID (NCBI) 338
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP04114
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).