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验证数据展示

  • WB analysis using 27751-1-AP

    WB analysis using 27751-1-AP

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 27751-1-AP (APOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27751-1-AP

    WB analysis using 27751-1-AP

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 27751-1-AP (APOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27751-1-AP

    IHC staining of mouse liver using 27751-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27751-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOB antibody included
  • IHC staining of mouse liver using 27751-1-AP

    IHC staining of mouse liver using 27751-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27751-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOB antibody included

APOB Polyclonal antibody

APOB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27751-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Apolipoprotein B, Apo B, Apo B-100, ApoB100, ApoB48

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® APOB Ready-To-Use IHC Kit. APOB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27751-1-AP targets APOB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26773

Product name: Recombinant human APOB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-220 aa of NM_000384

Sequence: MAGARAEEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKDEPTYILNIKRGIISALLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKGNVATEISTERDLGQCDRFKPIRT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein B (including Ag(x) antigen)
别名 Apolipoprotein B, Apo B, Apo B-100, ApoB100, ApoB48
计算分子量 516 kDa
观测分子量150-250 kDa, 400-520 kDa
GenBank蛋白编号NM_000384
基因名称 APOB
Gene ID (NCBI) 338
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04114
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. Unlike humans and rabbits, rats and mice secrete both apoB-100- and apoB-48-containing lipoproteins from the liver as a result of the hepatic expression of APOBEC-1 (PMID: 9633945). This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).

实验方案

Product Specific Protocols
IHC protocol for APOB antibody 27751-1-APDownload protocol
WB protocol for APOB antibody 27751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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