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验证数据展示

  • WB analysis using 31265-1-AP

    WB analysis using 31265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31265-1-AP (Apolipoprotein AI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31265-1-AP

    WB analysis using 31265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31265-1-AP (Apolipoprotein AI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 31265-1-AP

    IF Staining of HeLa using 31265-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Apolipoprotein AI antibody (31265-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31265-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

APOA1, apolipoprotein A I, APOA 1, ApoA I, ApoAI

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, mouse lung tissue, mouse spleen tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy APOA1 Ready-To-Use IHC Kit. APOA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31265-1-AP targets Apolipoprotein AI in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg0757

Product name: Recombinant Human APOA1 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 25-267 aa of BC005380

Sequence: DEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein A-I
别名 APOA1, apolipoprotein A I, APOA 1, ApoA I, ApoAI
计算分子量 31 kDa
观测分子量26 kDa
GenBank蛋白编号BC005380
基因名称 APOA1
Gene ID (NCBI) 335
RRIDAB_3669923
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP02647
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

实验方案

Product Specific Protocols
IF protocol for Apolipoprotein AI antibody 31265-1-APDownload protocol
WB protocol for Apolipoprotein AI antibody 31265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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