验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, Raji cells, mouse heart tissue, HEK-293T cells, HeLa cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83246-6-RR targets APCDD1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | APCDD1 fusion protein Ag10303 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | adenomatosis polyposis coli down-regulated 1 |
| 别名 | Protein APCDD1, FP7019, DRAPC1, B7323, Adenomatosis polyposis coli down-regulated 1 protein |
| 计算分子量 | 514 aa, 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC053324 |
| 基因名称 | APCDD1 |
| Gene ID (NCBI) | 147495 |
| RRID | AB_3670921 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q8J025 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
APCDD1(adenomatosis polyposis coli down-regulated 1) is a membrane-bound glycoprotein conserved during vertebrate evolution that regulates important biological processes controlled by Wnt signaling (PMID: 26170146). APCDD1 is a membrane-bound glycoprotein that is abundantly expressed in human HFs (hair follicles) and can interact in vitro with WNT3A and LRP5, two essential components of Wnt signaling (PMID: 20393562). APCDD1 may be modified by glycosylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APCDD1 antibody 83246-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |