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验证数据展示

  • WB analysis using 83246-6-RR (same clone as 83246-6-PBS)

    WB analysis using 83246-6-RR (same clone as 83246-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83246-6-PBS in a different storage buffer formulation.

  • WB analysis using 83246-6-RR (same clone as 83246-6-PBS)

    WB analysis using 83246-6-RR (same clone as 83246-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83246-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83246-6-RR

    Affinity and Kinetic Characterization of 83246-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83246-6-RR against Human APCDD1 were performed. The affinity constant is 0.634 nM.

APCDD1 Recombinant monoclonal antibody, PBS Only

APCDD1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83246-6-PBS

产品说明书

CloneNo. 240085C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Protein APCDD1, FP7019, DRAPC1, B7323, Adenomatosis polyposis coli down-regulated 1 protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83246-6-PBS targets APCDD1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10303

Product name: Recombinant human APCDD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 141-496 aa of BC053324

Sequence: TEADYQLHNVQVICHTEAVAEKLGQQVNRTCPGFLADGGPWVQDVAYDLWREENGCECTKAVNFAMHELQLIRVEKQYLHHNLDHLVEELFLGDIHTDATQRMFYRPSSYQPPLQNAKNHDHACIACRIIYRSDEHHPPILPPKADLTIGLHGEWVSQRCEVRPEVLFLTRHFIFHDNNNTWEGHYYHYSDPVCKHPTFSIYARGRYSRGVLSSRVMGGTEFVFKVNHMKVTPMDAATASLLNVFNGNECGAEGSWQVGIQQDVTHTNGCVALGIKLPHTEYEIFKMEQDARGRYLLFNGQRPSDGSSPDRPEKRATSYQMPLVQCASSSPRAEDLAEDSGSSLYGRAPGRHTWSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adenomatosis polyposis coli down-regulated 1
别名 Protein APCDD1, FP7019, DRAPC1, B7323, Adenomatosis polyposis coli down-regulated 1 protein
计算分子量 514 aa, 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC053324
基因名称 APCDD1
Gene ID (NCBI) 147495
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8J025
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APCDD1(adenomatosis polyposis coli down-regulated 1) is a membrane-bound glycoprotein conserved during vertebrate evolution that regulates important biological processes controlled by Wnt signaling (PMID: 26170146). APCDD1 is a membrane-bound glycoprotein that is abundantly expressed in human HFs (hair follicles) and can interact in vitro with WNT3A and LRP5, two essential components of Wnt signaling (PMID: 20393562). APCDD1 may be modified by glycosylation.

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