×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of human brain using 13177-1-AP

    WB analysis of human brain using 13177-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13177-1-AP (APBB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13177-1-AP

    IHC staining of human placenta using 13177-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APBB2 antibody included
  • IHC staining of human stomach cancer using 13177-1-AP

    IHC staining of human stomach cancer using 13177-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APBB2 antibody included
  • IHC staining of mouse stomach using 13177-1-AP

    IHC staining of mouse stomach using 13177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APBB2 antibody included
  • IF Staining of HeLa using 13177-1-AP

    IF Staining of HeLa using 13177-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13177-1-AP (APBB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse kidney using 13177-1-AP

    IHC staining of mouse kidney using 13177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APBB2 antibody included
  • IHC staining of mouse pancreas using 13177-1-AP

    IHC staining of mouse pancreas using 13177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APBB2 antibody included
  • IHC staining of rat kidney using 13177-1-AP

    IHC staining of rat kidney using 13177-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APBB2 antibody included

APBB2 Polyclonal antibody

APBB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13177-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

FE65L, FE65L1, A4, Amyloid-beta, Protein Fe65 like 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman placenta tissue, human stomach cancer tissue, mouse kidney tissue, mouse pancreas tissue, mouse stomach tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy APBB2 Ready-To-Use IHC Kit. APBB2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13177-1-AP targets APBB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3844

Product name: Recombinant human APBB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 386-736 aa of BC027946

Sequence: SCSINSDPEAKCFAVRSLGWVEMAEEDLAPGKSSVAVNNCIRQLSYCKNDIRDTVGIWGEGKDMYLILENDMLSLVDPMDRSVLHSQPIVSIRVWGVGRDNGRDFAYVARDKDTRILKCHVFRCDTPAKAIATSLHEICSKIMAERKNAKALACSSLQERANVNLDVPLQDFPTPKTELVQKFHVQYLGMLPVDKPVGMDILNSAIENLMTSSNKEDWLSVNMNVADATVTVISEKNEEEVLVECRVRFLSFMGVGKDVHTFAFIMDTGNQRFECHVFWCEPNAGNVSEAVQAACMLRYQKCLVARPPSQKVRPPPPPADSVTRRVTTNVKRGVLSLIDTLKQKRPVTEMP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 amyloid beta (A4) precursor protein-binding, family B, member 2
别名 FE65L, FE65L1, A4, Amyloid-beta, Protein Fe65 like 1
计算分子量 83 kDa
观测分子量 83-95 kDa
GenBank蛋白编号BC027946
基因名称 APBB2
Gene ID (NCBI) 323
RRIDAB_10638434
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92870
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for APBB2 antibody 13177-1-APDownload protocol
IHC protocol for APBB2 antibody 13177-1-APDownload protocol
WB protocol for APBB2 antibody 13177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...