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验证数据展示

  • WB analysis using 27355-1-AP (same clone as 27355-1-PBS)

    WB analysis using 27355-1-AP (same clone as 27355-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 27355-1-AP (same clone as 27355-1-PBS)

    IHC staining of human kidney using 27355-1-AP (same clone as 27355-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27355-1-AP (AP50 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 27355-1-AP (same clone as 27355-1-PBS)

    IHC staining of human kidney using 27355-1-AP (same clone as 27355-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27355-1-AP (AP50 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 27355-1-AP (same clone as 27355-1-PBS)

    IP experiment of mouse brain using 27355-1-AP (same clone as 27355-1-PBS)

    IP result of anti-AP50 (IP:27355-1-AP, 4ug; Detection:27355-1-AP 1:1000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 27355-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AP50 Polyclonal antibody, PBS Only

AP50 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27355-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

AP2M1, Adaptin mu2, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27355-1-PBS targets AP50 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26059

Product name: Recombinant human AP50 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-110 aa of BC013796

Sequence: MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 2, mu 1 subunit
别名 AP2M1, Adaptin mu2, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu
计算分子量 433 aa, 49 kDa
观测分子量 50 kDa
GenBank蛋白编号BC013796
基因名称 AP50
Gene ID (NCBI) 1173
RRIDAB_2880853
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CW1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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