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验证数据展示

  • WB analysis using 32945-1-AP

    WB analysis using 32945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32945-1-AP (AP3S1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 32945-1-AP

    IF Staining of mouse kidney using 32945-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using AP3S1 antibody (32945-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AP3S1 Polyclonal antibody

AP3S1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 32945-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF-P, ELISA

别名

Adaptor-related protein complex 3 subunit sigma-1, AP-3 complex subunit sigma-1, AP-3 complex subunit sigma-3A, CLAPS3, Clathrin-associated/assembly/adaptor protein, small 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32945-1-AP targets AP3S1 in WB, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37523

Product name: Recombinant human AP3S1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-193 aa of BC000804

Sequence: MIKAILIFNNHGKPRLSKFYQPYSEDTQQQIIRETFHLVSKRDENVCNFLEGGLLIGGSDNKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHVDKVHNILAEMVMGGMVLETNMNEIVTQIDAQNKLEKSEAGLAGAPARAVSAVKNMNLPEIPRNINIGDISIKVPNLPSFK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 3, sigma 1 subunit
别名 Adaptor-related protein complex 3 subunit sigma-1, AP-3 complex subunit sigma-1, AP-3 complex subunit sigma-3A, CLAPS3, Clathrin-associated/assembly/adaptor protein, small 3
计算分子量 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC000804
基因名称 AP3S1
Gene ID (NCBI) 1176
RRIDAB_3742800
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ92572
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP-3 complex subunit sigma-1 (AP3S1) is a component of the heterotetrameric adaptor protein complex-3 (AP-3), which plays a central role in intracellular protein trafficking (PMID: 34565296). AP3S1 is also a potential pan-cancer oncogene and plays an essential role in tumorigenesis and cancer immunity. Elevated expression of AP3S1 indicates an immunosuppressive microenvironment and can be used as a potential prognostic biomarker and a target for immunotherapy (PMID: 35874816; 38609993).

实验方案

Product Specific Protocols
IF protocol for AP3S1 antibody 32945-1-APDownload protocol
WB protocol for AP3S1 antibody 32945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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