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验证数据展示

  • WB analysis of Hacat cells (siRNA) using 11925-1-AP

    WB analysis of Hacat cells (siRNA) using 11925-1-AP

    WB result of AP3M2 antibody (11925-1-AP; 1:500) with siRNA cell lysate.

  • WB analysis of human brain using 11925-1-AP

    WB analysis of human brain using 11925-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11925-1-AP (AP3M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11925-1-AP

    IP experiment of mouse brain using 11925-1-AP

    IP result of anti-AP3M2 (IP:11925-1-AP, 4ug; Detection:11925-1-AP 1:300) with mouse brain tissue lysate 3200 ug.

  • IHC staining of mouse brain using 11925-1-AP

    IHC staining of mouse brain using 11925-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11925-1-AP (AP3M2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse brain using 11925-1-AP

    IHC staining of mouse brain using 11925-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11925-1-AP (AP3M2 Antibody) at dilution of 1:50 (under 40x lens).

  • Featured Product
  • KD/KO验证

AP3M2 Polyclonal antibody

AP3M2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11925-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

AP 3 complex subunit mu 2, AP3M2, AP47B, CLA20, HA1 47 kDa subunit homolog 2, Mu3B adaptin, P47B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, Hacat cells (siRNA)
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11925-1-AP targets AP3M2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AP3M2 fusion protein Ag2572 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 3, mu 2 subunit
别名 AP 3 complex subunit mu 2, AP3M2, AP47B, CLA20, HA1 47 kDa subunit homolog 2, Mu3B adaptin, P47B
计算分子量 418 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC056257
基因名称 AP3M2
Gene ID (NCBI) 10947
RRIDAB_10640438
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53677
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP3M2 is a 47-kDa protein that belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. AP-3 is implicated in the transport of lysosomal membrane proteins.

实验方案

Product Specific Protocols
WB protocol for AP3M2 antibody 11925-1-APDownload protocol
IHC protocol for AP3M2 antibody 11925-1-APDownload protocol
IP protocol for AP3M2 antibody 11925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols