×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 12114-1-AP (same clone as 12114-1-PBS)

    WB analysis using 12114-1-AP (same clone as 12114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12114-1-AP (AP3M1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12114-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 12114-1-AP (same clone as 12114-1-PBS)

    IHC staining of mouse cerebellum using 12114-1-AP (same clone as 12114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12114-1-AP (AP3M1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12114-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 12114-1-AP (same clone as 12114-1-PBS)

    IHC staining of mouse cerebellum using 12114-1-AP (same clone as 12114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12114-1-AP (AP3M1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12114-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12114-1-AP (same clone as 12114-1-PBS)

    IF Staining of HeLa using 12114-1-AP (same clone as 12114-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AP3M1 antibody (12114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12114-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AP3M1 Polyclonal antibody, PBS Only

AP3M1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12114-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

12114-1-PBS targets AP3M1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2759

Product name: Recombinant human AP3M1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 219-418 aa of BC026232

Sequence: SLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHSISFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLTWDVGKITPQKLPSLKGLVNLQSGAPKPEENPSLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYVTKAGKFQVRT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 3, mu 1 subunit
别名
计算分子量 418 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC026232
基因名称 AP3M1
Gene ID (NCBI) 26985
RRIDAB_2289629
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2T2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

||
New chat

Able™

正在加载，请稍候...