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验证数据展示

  • WB analysis using 68349-1-Ig

    WB analysis using 68349-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68349-1-Ig (AP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68349-1-Ig

    WB analysis of HeLa using 68349-1-Ig

    WB result of AP2B1 antibody (68349-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP2B1 transfected HeLa cells.

  • IHC staining of rat brain using 68349-1-Ig

    IHC staining of rat brain using 68349-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68349-1-Ig (AP2B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AP2B1 antibody included
  • IHC staining of rat brain using 68349-1-Ig

    IHC staining of rat brain using 68349-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68349-1-Ig (AP2B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AP2B1 antibody included
  • IF Staining of HeLa using 68349-1-Ig

    IF Staining of HeLa using 68349-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AP2B1 antibody (68349-1-Ig, Clone: 1A3B6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CoraLite®594-Phalloidin (red).

  • KD/KO Validated

AP2B1 Monoclonal antibody

AP2B1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68349-1-Ig

产品说明书

CloneNo. 1A3B6
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA

别名

Adaptor protein complex AP-2 subunit beta, Adaptor-related protein complex 2 subunit beta, ADTB2, AP 2 complex subunit beta, AP-2 complex subunit beta

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig cerebellum tissue, HeLa cells, rabbit cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AP2B1 Ready-To-Use IHC Kit. AP2B1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68349-1-Ig targets AP2B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性mouse
免疫原

CatNo: Ag8264

Product name: Recombinant human AP2B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 598-951 aa of BC006201

Sequence: GSTDAGDSPVGTTTATNLEQPQVIPSQGDLLGDLLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLLGSDLGGGIGGSPAVGQSFIPSSVPATFAPSPTPAVVSSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQFNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLFVEDGKMERQVFLATWKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNGIWILAELRIQPGNPNYTLSLKCRAPEVSQYIYQVYDSILKN

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 adaptor-related protein complex 2, beta 1 subunit
别名 Adaptor protein complex AP-2 subunit beta, Adaptor-related protein complex 2 subunit beta, ADTB2, AP 2 complex subunit beta, AP-2 complex subunit beta
计算分子量 105 kDa
观测分子量100-115 kDa
GenBank蛋白编号BC006201
基因名称 AP2B1
Gene ID (NCBI) 163
RRIDAB_3085072
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP63010
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in cargo selection by recognizing sorting signals in the cytoplasmic tails of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.

实验方案

Product Specific Protocols
IF protocol for AP2B1 antibody 68349-1-IgDownload protocol
IHC protocol for AP2B1 antibody 68349-1-IgDownload protocol
WB protocol for AP2B1 antibody 68349-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Commun

Resolving endogenous protein organization in cells with nanometer resolution.

Authors - Janna Eilts
View Article
mouseWB

Cell Mol Life Sci

ELAPOR1 mediated vesicle traffic is required for acrosome biogenesis and male fertility in mice.

Authors - Runze Ding
View Article
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