AP2A1 Polyclonal antibody
AP2A1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HEK-293 cells, MCF-7 cells, SKOV-3 cells, SH-SY5Y cells, mouse kidney tissue, rat kidney tissue, mouse kidney cells, rat kidney cells |
Planning an IHC experiment? We recommend our IHCeasy AP2A1 Ready-To-Use IHC Kit. AP2A1 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:12000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30030-1-AP targets AP2A1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, ELISA |
Tested Reactivity | Human, mouse, rat |
Immunogen | AP2A1 fusion protein Ag32232 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | adaptor-related protein complex 2, alpha 1 subunit |
Synonyms | ADTAA, Alpha adaptin A, Alpha1 adaptin, AP 2 complex subunit alpha 1, AP2 ALPHA, AP2A1, CLAPA1 |
Calculated Molecular Weight | 977 aa, 108 kDa |
Observed Molecular Weight | 108 kDa |
GenBank Accession Number | BC014214 |
Gene Symbol | AP2A1 |
Gene ID (NCBI) | 160 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95782 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.
实验方案
Product Specific Protocols | |
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WB protocol for AP2A1 antibody 30030-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |