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验证数据展示

  • WB analysis of HEK-293 using 87859-2-RR

    WB analysis of HEK-293 using 87859-2-RR

    WB result of AP1B1 antibody (87859-2-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP1B1 transfected HEK-293 cells.

  • WB analysis using 87859-2-RR

    WB analysis using 87859-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87859-2-RR (AP1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 87859-2-RR

    IP experiment of HEK-293 using 87859-2-RR

    IP result of anti-AP1B1 (IP:87859-2-RR, 4ug; Detection:87859-2-RR 1:3000) with HEK-293 cells lysate 1560 ug.

  • IF Staining of HepG2 using 87859-2-RR

    IF Staining of HepG2 using 87859-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AP1B1 antibody (87859-2-RR, Clone: 253524D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity of 87859-2-RR

    Affinity of 87859-2-RR

    Biolayer interferometry (BLI) kinetic assays of 87859-2-RR against Human AP1B1 were performed. The affinity constant is 0.356 nM.

  • KD/KO Validated

AP1B1 Recombinant monoclonal antibody

AP1B1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 87859-2-RR

产品说明书

CloneNo. 253524D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, ADTB1, AP 1 complex subunit beta 1, AP-1 complex subunit beta-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, HEK-293 cells, HeLa cells, A431 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87859-2-RR targets AP1B1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10417

Product name: Recombinant human AP1B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 671-919 aa of BC046242

Sequence: NFVAPPTAAVPANLGAPIGSGLSDLFDLTSGVGTLSGSYVAPKAVGSISMDLQLTNKALQVMTDFAIQFNRNSFGLAPAAPLQVHAPLSPNQTVEISLPLSTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHILFVEDGKMDRQMFLATWKDIPNENEAQFQIRDCPLNAEAASSKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGIWVLAELRIQPGNPSCTLSLKCRAPEVSQHVYQAYETILKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adaptor-related protein complex 1, beta 1 subunit
别名 Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, ADTB1, AP 1 complex subunit beta 1, AP-1 complex subunit beta-1
计算分子量 919 aa, 101 kDa
观测分子量 101 kDa
GenBank蛋白编号BC046242
基因名称 AP1B1
Gene ID (NCBI) 162
RRIDAB_3745793
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ10567
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

实验方案

Product Specific Protocols
IF protocol for AP1B1 antibody 87859-2-RRDownload protocol
IP protocol for AP1B1 antibody 87859-2-RRDownload protocol
WB protocol for AP1B1 antibody 87859-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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