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验证数据展示

  • WB analysis using 87859-1-RR (same clone as 87859-1-PBS)

    WB analysis using 87859-1-RR (same clone as 87859-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87859-1-RR (AP1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87859-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 87859-1-RR (same clone as 87859-1-PBS)

    WB analysis of HEK-293 using 87859-1-RR (same clone as 87859-1-PBS)

    WB result of AP1B1 antibody (87859-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP1B1 transfected HEK-293 cells. This data was developed using the same antibody clone with 87859-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 87859-1-RR (same clone as 87859-1-PBS)

    IHC staining of human colon using 87859-1-RR (same clone as 87859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 87859-1-RR (AP1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87859-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 87859-1-RR (same clone as 87859-1-PBS)

    IHC staining of human colon using 87859-1-RR (same clone as 87859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 87859-1-RR (AP1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87859-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 87859-1-RR (same clone as 87859-1-PBS)

    IF Staining of HepG2 using 87859-1-RR (same clone as 87859-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using AP1B1 antibody (87859-1-RR, Clone: 253524A11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87859-1-PBS in a different storage buffer formulation.

  • Affinity of 87859-1-RR

    Affinity of 87859-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87859-1-RR against Human AP1B1 were performed. The affinity constant is 11.0 nM.

AP1B1 Recombinant monoclonal antibody, PBS Only

AP1B1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87859-1-PBS

产品说明书

CloneNo. 253524A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, ADTB1, AP 1 complex subunit beta 1, AP-1 complex subunit beta-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87859-1-PBS targets AP1B1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10417

Product name: Recombinant human AP1B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 671-919 aa of BC046242

Sequence: NFVAPPTAAVPANLGAPIGSGLSDLFDLTSGVGTLSGSYVAPKAVGSISMDLQLTNKALQVMTDFAIQFNRNSFGLAPAAPLQVHAPLSPNQTVEISLPLSTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHILFVEDGKMDRQMFLATWKDIPNENEAQFQIRDCPLNAEAASSKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGIWVLAELRIQPGNPSCTLSLKCRAPEVSQHVYQAYETILKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adaptor-related protein complex 1, beta 1 subunit
别名 Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, ADTB1, AP 1 complex subunit beta 1, AP-1 complex subunit beta-1
计算分子量 919 aa, 101 kDa
观测分子量 101 kDa
GenBank蛋白编号BC046242
基因名称 AP1B1
Gene ID (NCBI) 162
RRIDAB_3745792
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ10567
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. (PMID: 41117250)

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