验证数据展示
经过测试的应用
| Positive ELISA detected in | peptide |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24493-1-AP targets AP repeat in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | AP repeat |
| 别名 | AP repeat, APAPAPAPA, C9RAN, GGGCC, poly AP |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2879572 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "AP repeat" sequence. This antibody detects the APAPAPAPA peptide with dilution 1:32,000 in Elisa.
发表文章
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Increased prevalence of granulovacuolar degeneration in C9orf72 mutation. | ||
Acta Neuropathol Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers. | ||
Acta Neuropathol Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy. | ||
Hum Mol Genet Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size. | ||
J Cell Sci SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72. | ||
Life Sci Alliance C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons. |
